replication in prokaryotes and eukaryotes slideshare

DNA replication is a very important and complex process in living organisms upon which all life depends. In E. coli, which has a single origin of replication on its one chromosome (as do most prokaryotes), it is approximately 245 base pairs long and is rich in AT sequences. (SV40 virus, causes cancer, is model for eukaryotic replication) Replication occurs bidirectionally with RNA primers. These are equivalent to the origin of replication in E. coli. Eukaryotic machinery is generally similar to that of E. coli. It occurs only in the S phase and at many chromosomal origins. A primer is required to initiate synthesis, which is then extended by DNA polymerase as it adds nucleotides one by one to the growing chain. Unlike prokaryotes, most eukaryotes are multicellular organisms, except for the unicellular eukaryotes such as yeast, flagellates, and ciliates. Features of Prokaryotic DNA Replication In other eukaryotes, like humans, there does not appear to be a consensus sequence for their origins of replication. They are known as pol α, pol β, pol γ, pol δ, and pol ε. It is the second step in genetic expression in which the ribosomes decodes the information present in mRNA to synthesize proteins according the sequence of codons present in them with different amino acids. Replication in eukaryotes starts at multiple origins of replication. The main difference between prokaryotic and eukaryotic DNA replication is that prokaryotic DNA replication occurs through a single origin of replication whereas eukaryotic DNA replication occurs through … DNA Replication in Prokaryotes Vs. Eukaryotes Location. Escherichia coli has 4.6 million base pairs in a single circular chromosome, and all of it gets replicated in approximately 42 minutes, starting from a single origin of replication and proceeding around the chromosome in both directions. The mechanism is quite similar to that in prokaryotes. Replication in eukaryotes starts at multiple origins of replication. Replication in eukaryotes starts at multiple origins of replication. DNA replication uses a semi-conservative method that results in a double-stranded DNA with one parental strand and a new daughter strand. However, the initiation process is more complex in eukaryotes than prokaryotes. Replication in eukaryotes. The rate of replication is approximately 100 nucleotides per second, much slower than prokaryotic replication. All DNA polymerases possess a 5′->3′ polymerase activity. 3 Eukaryotic Replication Machinery. Eukaryotic genomes are much more complex and larger in size than prokaryotic genomes. DNA polymerase types. Prokaryotic vs Eukaryotic Translation. The number of DNA polymerases in eukaryotes is much more than in prokaryotes: 14 are known, of which five are known to have major roles during replication and have been well studied. Prokaryotic DNA replication is often studied in the model organism coli, but all other prokaryotes show many similarities. ... DNA Replication (3) Ecology (42) Ecology & Environmental Science (1) Ecology MCQ (7) Ecology PPT (5) Embryology (4) Endocrinology (3) Endocrinology (4) Translation is the process of protein synthesis in the cells. A pre-replication complex is made with other initiator proteins. The replication occurs in … Summary. In eukaryotes, there are multiple origin of replication present. A primer is required to initiate synthesis, which is then extended by DNA polymerase as it adds nucleotides one by one to the growing chain. They are known as pol α, pol β, pol γ, pol δ, and pol ε. The number of DNA polymerases in eukaryotes is much more than in prokaryotes: 14 are known, of which five are known to have major roles during replication and have been well studied. 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